Coding

Part:BBa_M31234

Designed by: Kate Broadbent   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2008-05-13)


Calcium channel-apoaequorin fusion

This part encodes for the homo sapiens neuronal voltage-gated calcium ion channel gamma-3 subunit linked to Aequorea victoria apoaequorin. The ion channel is expressed in the membrane of the presynaptic cleft, with an apoaequorin linked to the C-terminal of the channel. If coelenterazine is present within the neuron, it binds to the apoaequorin along with oxygen. When an action potential reaches the modified ion channel, calcium ions flood in to the cell, and 2 or 3 of them bind to the apoaequorin. When the calcium ions bind, the coelenterazine is oxidized, and light in the form of bioluminescence is emitted as a byproduct. This part, when used in tandem with coelenterazine will show the activity of neurons by bioluminescing whenever an action potential reaches the synapse.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1717
    Illegal BamHI site found at 964
    Illegal BamHI site found at 1614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 734


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Parameters
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