Composite

Part:BBa_M31200

Designed by: Robert Warden   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)

M13.1 Genome

General Info

This is a refactoring of the M13 genome between the natural HpaI site in Gene II and the BamHI site in Gene III.

Redesign Elements

Specifics of redesign for each part can be found on the "Part Design" section of that part. The following are general redesign concepts used in the M13.1 refactoring.

Decompression

All overlapping genes and promoters have been separated out into a linear sequence of Gene-Promoter-RBS-Gene...

Codon Optimization

All genes completely contained within the region have been codon optimized.

The optimization was run by the [http://www.evolvingcode.net/codon/sgd/index.php Synthetic Gene Designer]. The codon bias was used according to Escherichia coli [gbbct]: 14553.

Restriction Sites

Unique restriction sites were added after each ORF. Gene VIII also has a FspI site added overlapping the start codon, so that fusion protiens can be quickly produced.

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