Measurement

Part:BBa_M30009

Designed by: natalie kuldell   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2005-12-04)


-- No description --

Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lambda cI gene. The cI protein represses expression of mRFP (M30003). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1684
    Illegal AgeI site found at 1796
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/measurement/uncategorized
Parameters
None