Generator

Part:BBa_M30001

Designed by: natalie kuldell   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2005-11-24)


-- No description --

P22 c2 inverter (Q04530) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04530 fragment with four-part inverter. Cells carrying M30001 are red, however because PCR was used for cloning there may be silent mutations in the insert. M30001 is clone #1. A second clone will be saved as M30002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1510
    Illegal AgeI site found at 1622
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 184


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Categories
//classic/generator/uncategorized
Parameters
None