Coding

Part:BBa_M1730

Designed by: Susan Frisby   Group: UtahState_BE5930_-_S11   (2013-04-20)


pBad with optimized hepcidin along with a histag

A part created for a class project, all based on theory. Inducible expression of the pBad promoter of the codon optimized hepcidin protein along with a histag for purification

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None