Reporter

Part:BBa_M1729

Designed by: Susan Frisby   Group: UtahState_BE5930_-_S11   (2013-04-19)


GFP reporter expression after induction with pBAD and hepcidin.

This part is created in theory for a synthetic bio class. It will test the induction of GFP with hepcidin using the pBAD promoter to see the optimial concentration of arabinose needed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2207
    Illegal SapI site found at 961


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Categories
Parameters
None