Composite

Part:BBa_M1727

Designed by: Richard Decker   Group: UtahState_BE5930_-_S11   (2013-04-13)


Promoter + RBS + Hepcidin + Silk + His-Tag

This part was designed for a class and is not available. It contains a pBAD promoter, a standard RBS, the coding region for Hepcidin, a 6x repeat of spider silk, and a 10x histidine tag, followed by a double stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None