Composite

Part:BBa_M1726

Designed by: Susan Frisby   Group: UtahState_BE5930_-_S11   (2013-04-03)


pBAD + hepcidin + histag

This part is for a class project. This part is based on theory. This part contains the pBAD promoter which will allow us to control expression of our protein of interest, hepcidin. Adding a Histag on the end will be used for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None