Composite

Part:BBa_M1705

Designed by: S. Ivan Bartlett   Group: UtahState_BE5930_-_S11   (2013-04-19)


Inulosucrase/scrR/scrT complete operon

This is a composite part built from part M1704 (promoter+RBS+scrR+terminator) and part M1703 (promoter+RBS+inulosucrase+RBS+scrT+terminator). With this combination it is now a complete operon for the increased uptake of sucrose into the cell and conversion of sucrose to inulin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2560
    Illegal BamHI site found at 4108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1007
    Illegal AgeI site found at 1138
    Illegal AgeI site found at 3275
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3621
    Illegal BsaI.rc site found at 3360
    Illegal BsaI.rc site found at 3423
    Illegal BsaI.rc site found at 4415


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Categories
Parameters
None