Coding

Part:BBa_M1699

Designed by: S. Ivan Bartlett   Group: UtahState_BE5930_-_S11   (2013-04-15)


scrT

This gene sequence codes for the ScrT protein which serves as a symporter for sucrose into the cell. The sequence is originally from [Bifidobacterium animalis subsp. lactis]. The sequence is normally a part of a gene cluster containing scrT, scrP, and scrR. Only scrT was used specifically from these gene cluster in our project to increase the uptake of sucrose by the cell. The sequence present in this part has been optimized for use in Lactobacillus johnsonii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 797


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