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Part:BBa_M1541

Designed by: Brian Smith   Group: UtahState_BE5930_-_S11   (2012-04-21)


Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL3. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4439
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2106
    Illegal BglII site found at 2769
    Illegal BamHI site found at 4958
    Illegal XhoI site found at 4859
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 4275
    Illegal AgeI site found at 4332
  • 1000
    COMPATIBLE WITH RFC[1000]


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