Composite

Part:BBa_M1535

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-20)


Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2246
    Illegal BglII site found at 2909
    Illegal BamHI site found at 5098
    Illegal XhoI site found at 4999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1176
    Illegal AgeI site found at 496
    Illegal AgeI site found at 4415
    Illegal AgeI site found at 4472
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None