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Part:BBa_M1533

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-18)


Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2261
    Illegal BglII site found at 2924
    Illegal BglII site found at 6838
    Illegal BglII site found at 7501
    Illegal BamHI site found at 5327
    Illegal XhoI site found at 5228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 5768
    Illegal AgeI site found at 4430
    Illegal AgeI site found at 4487
    Illegal AgeI site found at 9007
    Illegal AgeI site found at 9064
  • 1000
    COMPATIBLE WITH RFC[1000]


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