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Part:BBa_M1530

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-17)


Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4768
    Illegal SpeI site found at 923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2241
    Illegal BglII site found at 2904
    Illegal BglII site found at 6798
    Illegal BglII site found at 7461
    Illegal BamHI site found at 5287
    Illegal XhoI site found at 5188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 923
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 5728
    Illegal AgeI site found at 4410
    Illegal AgeI site found at 4467
    Illegal AgeI site found at 8967
    Illegal AgeI site found at 9024
  • 1000
    COMPATIBLE WITH RFC[1000]


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