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Part:BBa_M1515

Designed by: Brian Smith   Group: UtahState_BE5930_-_S11   (2012-04-19)


Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL3. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 835
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4700
    Illegal SpeI site found at 835
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2153
    Illegal BglII site found at 2816
    Illegal BglII site found at 6730
    Illegal BglII site found at 7393
    Illegal BamHI site found at 5219
    Illegal XhoI site found at 5120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 835
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 835
    Illegal NgoMIV site found at 1083
    Illegal NgoMIV site found at 5660
    Illegal AgeI site found at 4322
    Illegal AgeI site found at 4379
    Illegal AgeI site found at 8899
    Illegal AgeI site found at 8956
  • 1000
    COMPATIBLE WITH RFC[1000]


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