Composite

Part:BBa_M1212

Designed by: Lindsey Stevens   Group: UtahState_BE5930_-_S11   (2011-04-25)


Kill Gene with CFP alternative

This part is used to kill an E. coli cell when turned on. The holin operates to lyse the cell if the cell is not maintained at 42 degrees Celsius or if tetracycline interacts with the repressor. The reporter CFP, glows if these conditions are met, marking the cell for death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2161
    Illegal NheI site found at 2184
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1766
    Illegal AgeI site found at 1836
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2437


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Categories
Parameters
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