Composite

Part:BBa_M1211

Designed by: Lindsey Stevens   Group: UtahState_BE5930_-_S11   (2011-04-25)


Kill Gene with RFP alternative

This part contains a kill gene that uses holin to produce holes in the cell wall and destroy the cell if the cell is below 42 degrees Celsius or in the presence of tetracycline. RFP is used as a reporter to visually alert the researcher to a cell which is marked for death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2144
    Illegal NheI site found at 2167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
    Illegal AgeI site found at 1749
    Illegal AgeI site found at 1819
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2420


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Categories
Parameters
None