Intermediate

Part:BBa_M1208

Designed by: Aaron Winder   Group: UtahState_BE5930_-_S11   (2011-04-25)

Kill gene intermediate - Holin/Autolysin portion

This part is an intermediate of the completed kill gene. It includes the tet-promoter, RBS, GFP, holin and autolysin portions and a double terminator. The tet-promoter is to ultimately be regulated by a separate part of the construct regulating cell survival.

The GFP portion is meant to give a warning system in case any mutation occurs in the critical functioning sections of the construct. Since it is expressed with the suicide portion of the construct, any glowing culture is expressing the kill gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1763
    Illegal AgeI site found at 1833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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Categories
Parameters
None