Part:BBa_M12068

BioBrick-Compatible Broad-Host-Range Vector pPMQAK1

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

This shuttle vector appears to be useful for the expression of BioBricks in multiple chassis, including non-Escherichia coli microbes. According to the authors:

"This vector contains the replicon of the broad-host-range plasmid RSF1010, designed for replication in different cyanobacterial, and even non-cyanobacterial, strains. The RSF1010 replicon of the IncQ group is well known for allowing plasmids to replicate in a large variety of gram negative bacteria, but there are examples of successful replication in gram positive bacteria as well. In fact, the replicative and conjugal abilities of IncQ type plasmids make them the most wide-spread bacterial replicons known. This includes, for example, bacteria from the genus Pseudomonas, cyanobacterial genera such as Synechococcus, Prochlorococcus and Agrobacterium tumefaciens."

• Please Note: I did not design this vector, and am not an expert on either its construction or its function. All information disclosed is based on my understanding of the vector and its potential usefulness, and there is a possibility some claims have been misrepresented. That said, to the best of my knowledge, this information is accurate.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1924
  Illegal suffix found in sequence at 2621
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1924
  Illegal SpeI site found at 2622
  Illegal PstI site found at 2636
  Illegal NotI site found at 1930
  Illegal NotI site found at 2629
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1924
  Illegal BamHI site found at 2609
  Illegal XhoI site found at 817
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1924
  Illegal suffix found in sequence at 2622
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1924
  Illegal XbaI site found at 1939
  Illegal SpeI site found at 2622
  Illegal PstI site found at 2636
  Illegal AgeI site found at 3681
  Illegal AgeI site found at 4082
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 963
  Illegal BsaI.rc site found at 2059