Intermediate

Part:BBa_M1206

Designed by: Andrea Olson   Group: UtahState_BE5930_-_S11   (2011-04-18)


Suicide Gene with GFP Tag

Suicide Gene that utilizes T4 Holin and Lysozymes to degrade the cell wall and perforate the inner membrane of the cell, resulting in the death of the cell. A T4 antiholin is present when the cell is not to be terminated. The GFP marker is in place to ensure that kill gene is working when below 42C. The suicide gene is controlled by a tetracycline repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2158
    Illegal NheI site found at 2181
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1763
    Illegal AgeI site found at 1833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724
    Illegal SapI site found at 2434


[edit]
Categories
Parameters
None