Composite

Part:BBa_M1205

Designed by: Andrea Olson   Group: UtahState_BE5930_-_S11   (2011-04-18)


Complete Part for ROSE Regulated Kill Gene

This gene is meant to be used as a regulatory gene to prevent accidental spread of any experiment cultures that may be produced, either in the lab or in DIY kits. It works by utilizing a temperature sensitive ROSE sequence that creates a hairpin structure that prevents translation of a tetracycline repressor. This allows for the suicide gene to be coded for and results in the death of the cell. When conditions are above 42C, the hairpin bonds are broken and the tetracycline repressor is now coded, repressing the kill switch and allowing for the continual growth of the culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3300
    Illegal NheI site found at 3323
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2905
    Illegal AgeI site found at 2975
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1866
    Illegal SapI site found at 3576


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Parameters
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