Part:BBa_M11421

Streptomyces Shuttle Vetor Thiostrepton Resistance

This is a shuttle vector to be used with E. coli and Streptomyces. It has thiostrepton resistance.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11868
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2075
  Illegal NotI site found at 2201
  Illegal NotI site found at 2294
  Illegal NotI site found at 3386
  Illegal NotI site found at 3415
  Illegal NotI site found at 4123
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 11868
  Illegal XhoI site found at 1559
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 11868
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 11868
  Plasmid lacks a suffix.
  Illegal XbaI site found at 11883
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 719
  Illegal NgoMIV site found at 1513
  Illegal NgoMIV site found at 2851
  Illegal NgoMIV site found at 3630
  Illegal NgoMIV site found at 4000
  Illegal NgoMIV site found at 5732
  Illegal NgoMIV site found at 5789
  Illegal AgeI site found at 603
  Illegal AgeI site found at 1794
  Illegal AgeI site found at 6577
  Illegal AgeI site found at 7386
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 4594
  Illegal BsaI site found at 5422
  Illegal BsaI.rc site found at 1503
  Illegal BsaI.rc site found at 5082
  Illegal BsaI.rc site found at 6380
  Illegal BsaI.rc site found at 10933
  Illegal SapI.rc site found at 241