Part:BBa_M11400

Integration Plasmid for Synechocystis sp. PCC 6803

This plasmid is designed to integrated into the Synechocystis sp. PCC 6803 genome. It contains an ampicillin resistance gene and an E. coli origin of replication, so BioBrick parts can be assembled in E. coli, then transformed into Synechocystis for genomic integration. In Synechocystis, transformed cells can be selected with kanamycin. M11400 is based on plasmid pIGA from Kunert et al. (2000), GenBank accession number AJ251839.1. The recombination sites correspond to the regions containing hypothetical proteins slr0338 and slr0168 in Synechocystis sp. PCC 6803.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1336
  Illegal XbaI site found at 1321
  Illegal SpeI site found at 1306
  Illegal PstI site found at 1292
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1336
  Illegal NheI site found at 1537
  Illegal NheI site found at 5952
  Illegal SpeI site found at 1306
  Illegal PstI site found at 1292
  Illegal NotI site found at 1119
  Illegal NotI site found at 1297
  Illegal NotI site found at 1313
  Illegal NotI site found at 1328
  Illegal NotI site found at 1548
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1336
  Illegal BglII site found at 2706
  Illegal BamHI site found at 2917
  Illegal BamHI site found at 5272
  Illegal XhoI site found at 30
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1336
  Illegal XbaI site found at 1321
  Illegal SpeI site found at 1306
  Illegal PstI site found at 1292
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1336
  Illegal XbaI site found at 1321
  Illegal SpeI site found at 1306
  Illegal PstI site found at 1292
  Illegal NgoMIV site found at 2041
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4247
  Illegal BsaI.rc site found at 2156