Device

Part:BBa_M11207

Designed by: Angela Dixon   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-22)


Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

Used in S. cerevisiae to monitor glucose and oxygen levels. It is composed of two BioBrick devices:

Device 1: The promoter, HXT7, is repressed in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Device 2: The promoter, mNarK , is activated under anaerobic condtions. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the RFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3375
    Illegal AgeI site found at 3487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168
    Illegal BsaI.rc site found at 2389
    Illegal SapI.rc site found at 136


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