Device

Part:BBa_M11204

Designed by: Angela Dixon   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-22)


HXT7 promoter + GFP to monitor glucose levels in S. cerevisiae

Used in S. cerevisiae to monitor glucose levels. The promoter, HXT7, is repressed in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168
    Illegal BsaI.rc site found at 2389
    Illegal SapI.rc site found at 136


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