Plasmid_Backbone

Part:BBa_M11201

Designed by: Angela Dixon   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-21)


pRS316 plasmid for S. cerevisiae in BioBrick format

Modified pRS316 plasmid for S. cerevisiae. Has selection for tryptophan negative, Ampicillin, and LacZ. Can be used to insert BioBricks into S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3349
    Illegal XbaI site found at 3355
    Illegal SpeI site found at 3373
    Illegal PstI site found at 3379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3349
    Illegal SpeI site found at 3373
    Illegal PstI site found at 3379
    Illegal NotI site found at 3341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3349
    Illegal BamHI site found at 3361
    Illegal XhoI site found at 3412
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3349
    Illegal XbaI site found at 3355
    Illegal SpeI site found at 3373
    Illegal PstI site found at 3379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3349
    Illegal XbaI site found at 3355
    Illegal SpeI site found at 3373
    Illegal PstI site found at 3379
    Illegal NgoMIV site found at 3000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2411
    Illegal BsaI.rc site found at 4785
    Illegal SapI site found at 3702
    Illegal SapI site found at 5806
    Illegal SapI.rc site found at 2258


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Categories
Parameters
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