Part:BBa_M11200

pRS426 plasmid vector for S. cerevisiae

Plasmid backbone used to put BioBricks into S. cerevisiae. Contains selection for uracil negative,ampicillin and LacZ.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2032
  Illegal XbaI site found at 2038
  Illegal SpeI site found at 2056
  Illegal PstI site found at 2062
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2032
  Illegal SpeI site found at 2056
  Illegal PstI site found at 2062
  Illegal NotI site found at 2068
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2032
  Illegal BamHI site found at 2050
  Illegal XhoI site found at 1999
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2032
  Illegal XbaI site found at 2038
  Illegal SpeI site found at 2056
  Illegal PstI site found at 2062
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2032
  Illegal XbaI site found at 2038
  Illegal SpeI site found at 2056
  Illegal PstI site found at 2062
  Illegal NgoMIV site found at 1656
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1065
  Illegal BsaI.rc site found at 3444
  Illegal SapI site found at 2361
  Illegal SapI.rc site found at 912