Part:BBa_M11076

NrtA: nitrate/nitrite binding protein coding region

NrtA is one of four polypeptides found in the high-affinity active nitrate transport system of Synechococcus sp. JA-3-3Ab. Specifically, NrtA is a periplasmic substrate-binding protein that has a specific and high binding affinity to nitrate and nitrite. The presence of NrtA is necessary for cell survival under conditions where nitrate is the primary available nitrogen source.

The presence of available nitrate is important for photosynthesis and growth. Understanding the components of the ABC nitrate/nitrite transport system and having the ability to manipulate these proteins could be of interest in many photosynthetic growth applications. Additionally, nitrate is a nutrient that is removed in wastewater treatment systems. The high binding affinity of this particular protein to nitrate could be useful in nitrate removal.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 878
  Illegal BamHI site found at 92
  Illegal BamHI site found at 313
  Illegal BamHI site found at 540
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 322
  Illegal NgoMIV site found at 532
  
• 1000
  COMPATIBLE WITH RFC[1000]