Part:BBa_M11058

pfaA gene for omega-3 fatty acid synthesis

This is a single open reading frame which contains multiple genes that codes for necessary proteins in the sysnthesis of omega-3 polyunsaturated fatty acids. Ecosapentaenoic acid is the PUFA that is synthesized using this organism. The proteins encoded within is this gene are for polyketide synthases (PKSs), which polymerize simple fatty acids into a large variety of different products, called polyketides, by successive decarboxylating Claisen condensations. PKSs can be divided into 2 groups, modular type I PKSs consisting of one or more large multifunctional proteins and iterative type II PKSs, complexes of several monofunctional subunits. This gene is to be used in sequence with pfaB, pfaC, and pfaD. By cloning these genes into the same organism, it should be able to produce omega-3 fatty acids.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1255
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4071
  Illegal BglII site found at 4464
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2856
  Illegal NgoMIV site found at 3927
  Illegal NgoMIV site found at 4215
  Illegal NgoMIV site found at 4521
  Illegal NgoMIV site found at 4830
  Illegal NgoMIV site found at 5268
  Illegal NgoMIV site found at 6177
  Illegal AgeI site found at 1102
  Illegal AgeI site found at 2947
  Illegal AgeI site found at 4239
  Illegal AgeI site found at 4639
  Illegal AgeI site found at 4875
  Illegal AgeI site found at 6249
  Illegal AgeI site found at 6385
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 2934