Composite

Part:BBa_M1032

Designed by: Misheka Wilson   Group: UtahState_BE5930_-_S11   (2011-04-27)


PhaA PhaB PhaC and zinc fingers

These are the three enzymes in the Pha catalytic enzymatic pathway and the zinc fingers that are solar fusion compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1860
    Illegal BglII site found at 3505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2248
    Illegal NgoMIV site found at 4486
    Illegal AgeI site found at 95
    Illegal AgeI site found at 1620
    Illegal AgeI site found at 2717
    Illegal AgeI site found at 2804
    Illegal AgeI site found at 2948
    Illegal AgeI site found at 3221
    Illegal AgeI site found at 4004
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 453
    Illegal SapI site found at 582


[edit]
Categories
Parameters
None