Composite

Part:BBa_M1023

Designed by: Miguel Suastegui   Group: UtahState_BE5930_-_S11   (2011-04-13)

RBS+"Blues" Zinc Finger+ phaC+ terminator

This composite part is composed by the "Blues" Zinc Finger fused to PhaC plus BBa_B1001 which is a terminator sequence. The designed part is compatible with the Silver fusion assembly standard. The codons were optimized for expression on E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal AgeI site found at 111
    Illegal AgeI site found at 198
    Illegal AgeI site found at 342
    Illegal AgeI site found at 615
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None