Composite

Part:BBa_M1021

Designed by: Miguel Suastegui   Group: UtahState_BE5930_-_S11   (2011-04-12)

RBS+ "Blues" Zinc Finger + PhaC

This composite part contains the ribosome binding site BBa_B0035, and the "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. The last two coding sequences are silver fusion compatible and optimized for expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal AgeI site found at 111
    Illegal AgeI site found at 198
    Illegal AgeI site found at 342
    Illegal AgeI site found at 615
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None