Composite
Part:BBa_M1021
Designed by: Miguel Suastegui Group: UtahState_BE5930_-_S11 (2011-04-12)
RBS+ "Blues" Zinc Finger + PhaC
This composite part contains the ribosome binding site BBa_B0035, and the "Blues" Zinc Finger fused to PhaC. PhaC is the last enzyme in the PHA synthesis pathway. It catalyzes the polymerization of hydroxyacyl-CoA substrates to yield PHAs. The last two coding sequences are silver fusion compatible and optimized for expression in E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 899
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1880
Illegal AgeI site found at 111
Illegal AgeI site found at 198
Illegal AgeI site found at 342
Illegal AgeI site found at 615
Illegal AgeI site found at 1398 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |