Coding

Part:BBa_M1019

Designed by: Michaela Hugie   Group: UtahState_BE5930_-_S11   (2011-04-19)


Silver Fusion Compatible PhaB gene with zif23-GCN4

The bioplastic enzyme PhaB has been combined with a zinc finger to increase binding. Also this sequence is silver fusion compatible and has a scar in between the Zif23-GCN4 and the PhaB enzyme. The optimal RBS has been attached at the beginning of the sequence

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None