Coding

Part:BBa_M1017

Designed by: Michaela Hugie   Group: UtahState_BE5930_-_S11   (2011-04-13)


Silver Fusion Compatible PhaB gene with zif23-GCN4

This sequence includes the RBS (with the best compatibility for the use of zinc fingers), PhaB enzyme, zif23-GCN4, and a silver fusion scar tissue sequence. The silver fusion scar tissue ensures the binding within a silver fusion vector. The zinc finger is there to help with the binding and translation of the PhaB enzyme. PhaB is a biodegradable plastic enzyme for polyhydroxylakanoate (PHB).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1328
    Illegal BglII site found at 2413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 1716
    Illegal NgoMIV site found at 2801
    Illegal AgeI site found at 1088
    Illegal AgeI site found at 2173
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None