Composite

Part:BBa_M1014

Designed by: Alex Hatch   Group: UtahState_BE5930_-_S11   (2011-04-19)


Complete construct for phasin secretion together with zinc finger binding sites

This construct is designed to exist in a plasmid separate from a plasmid coding for the zinc finger-fused enzymes involved in the PHB enzymatic pathway. This construct codes for phasin targeted for secretion by the Type I pathway as well as the Type I associated membrane proteins HlyB and HlyD. It also contains a repeated sequence that allows ordered binding of Zif268-HIV, Zif23-GCN 4, and "Blues" Zinc fingers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2145
    Illegal BglII site found at 2808
    Illegal BamHI site found at 4856
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
    Illegal AgeI site found at 4314
    Illegal AgeI site found at 4371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4708


[edit]
Categories
Parameters
None