Composite

Part:BBa_M1013

Designed by: Alex Hatch   Group: UtahState_BE5930_-_S11   (2011-04-19)


Complete Construct for Type I Phasin Secretion

This construct codes for phasin targeted for secretion with the HlyA signal peptide. It also codes for the HlyB and HlyD membrane proteins necessary for type I secretion. Coding regions are controlled under high levels of constitutive expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2145
    Illegal BglII site found at 2808
    Illegal BamHI site found at 4856
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
    Illegal AgeI site found at 4314
    Illegal AgeI site found at 4371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4708


[edit]
Categories
Parameters
None