Part:BBa_M10088

{˂TshA!} | N-terminal displaying Tsh Autotransporter

Autotransporters are mainly used for the translocation of large proteins or protein domains across the outer membrane. The autotransporter protein contains it own machinery, a transporter domain, to secrete its covalently attached, N-terminal passenger across the outer membrane. After secretion to the periplasm through normal SEC dependent pathways, the autotransporter portion inserts into the outer membrane as a Beta-barrel forming oligomeric pores, similar to porins. The covalently linked passenger peptide will pass through this self-formed beta-barrel folding progressively into its native configuration as it reaches the cell surface. The passenger peptide remains in a non-native conformation while it is in the cytoplasm and the periplasm.

Please see PMID: 15546669 for more information.

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This part has been successfully used to display a leucine zipper passenger peptide {GS5-IILK}
{GS5-IILK˃}{˂TshA!} device

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

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Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 856
  Illegal PstI site found at 1201
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 856
  Illegal PstI site found at 1201
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 856
  Illegal PstI site found at 1201
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 856
  Illegal PstI site found at 1201
  Illegal NgoMIV site found at 1342
  
• 1000
  COMPATIBLE WITH RFC[1000]