Part:BBa_M10088
{˂TshA!} | N-terminal displaying Tsh Autotransporter
Autotransporters are mainly used for the translocation of large proteins or protein domains across the outer membrane. The autotransporter protein contains it own machinery, a transporter domain, to secrete its covalently attached, N-terminal passenger across the outer membrane. After secretion to the periplasm through normal SEC dependent pathways, the autotransporter portion inserts into the outer membrane as a Beta-barrel forming oligomeric pores, similar to porins. The covalently linked passenger peptide will pass through this self-formed beta-barrel folding progressively into its native configuration as it reaches the cell surface. The passenger peptide remains in a non-native conformation while it is in the cytoplasm and the periplasm.
Please see PMID: 15546669 for more information.
This part has been successfully used to display a leucine zipper passenger peptide {GS5-IILK}
{GS5-IILK˃}{˂TshA!} device
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 856
Illegal PstI site found at 1201 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 856
Illegal PstI site found at 1201 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 856
Illegal PstI site found at 1201 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 856
Illegal PstI site found at 1201
Illegal NgoMIV site found at 1342 - 1000COMPATIBLE WITH RFC[1000]
None |