Coding

Part:BBa_M10086

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-01)

{˂CPG_L2!}

Circularly permutated OmpG with a backbone opening in loop 2 of the native protein.

Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.

CPG_L2 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {<CPG_L2!} and should be functionally displayed on the outer membrane of E.coli.

Streptavidin binding assay template
Growth curve assay template
Silver binding assay template
Autoaggregation assay template

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a{˂CPG_L2!}