Coding

Part:BBa_M10063

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-10)


{CPompX!}

Circularly permutated ompX.

Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.

CPompX is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {CPompX!} and should be functionally displayed on the outer membrane of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 175
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None