Part:BBa_M10053

{˂EILD˃}

This basic part codes for an alpha helix that is one coil of the coiled-coil structure of the KILR/EILD heterodimer. EILD is capable of forming homodimers. The competing states are the homodimer, unfolded, and aggregated states. When the two homodimers rearrange to form heterodimers, the free energy change is -8.7kcal/mol. The EILD is a designed protein that was made based on conformational free energy and potential energy functions. A use for the KILR/EILD heterodimer was not specified. Protein-protein interactions such as coiled-coils are crucial in signaling networks and multi-protein assembly.

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]