Part:BBa_M10048

{˂Inp-repeats˃} Ice Nucleation Protein (Inp) Repeats

http://www.ebi.ac.uk/interpro/IEntry?ac=IPR000258

The part encodes for the region of the ice-nucleation protein (Inp) containing motifs of 48-amino acid repeats, enabling the seeding of ice crystals. The endogenous function may have use in a surface display assay.

As an outer membrane protein, the full Inp has been previously validated for use in cell-surface display:

Jung HC, Lebeault JM, Pan JG. Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae. Nature Biotechnology. 16, 576-580 (1998).

Shimazu M, Mulchandani A, Chen W. Cell surface display of organophosphorus hydrolase using ice nucleation protein. Biotechnology Progress. 17, 76-80 (2001).

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal prefix found in sequence at 6
  Illegal BamHI site found at 2286
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal NgoMIV site found at 63
  Illegal NgoMIV site found at 1575
  Illegal NgoMIV site found at 1719
  Illegal NgoMIV site found at 1983
  Illegal NgoMIV site found at 2127
  Illegal NgoMIV site found at 2175
  Illegal AgeI site found at 1239
  
• 1000
  COMPATIBLE WITH RFC[1000]