Part:BBa_M10041
{a~INP˃} | C-terminal displaying Ice Nucleation Protein
Basic Part codes for Ice Nucleation Protein (INP) from Pseudomonas syringae genomic DNA. INP is a membrane-bound protein which allows host cells to nucleate crystallization in supercooled water. INP's have three structurally distinguished domains. The N-terminal domain is responsible for targeting the entire protein to the outer membrane and might be useful as a functional anchoring motif for cell surface protein display.
Please See PMID: 14705004 for more information
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 650
Illegal NgoMIV site found at 2162
Illegal NgoMIV site found at 2306
Illegal NgoMIV site found at 2570
Illegal NgoMIV site found at 2714
Illegal NgoMIV site found at 2762
Illegal NgoMIV site found at 2882
Illegal AgeI site found at 421
Illegal AgeI site found at 1826 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 596
Illegal BsaI.rc site found at 3342
Illegal SapI.rc site found at 242
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