Part:BBa_M10037

{˂KILR˃}

This basic part codes for an alpha helix which forms coiled-coil heterodimers with the alpha helix protein EILD. This part can be used in conjuction with EILD for binding based purification methods or or for binding based localization of proteins. This part includes no start and no stop codon and is meant to be used in translational fusion peptides.

The sequence was designed using a multi-state computational method, which attempted to optimize the free energy of KILR/EILD heterodimer formation relative to KILR and EILD homodimer formation. The calculated free energy change from the homodimeric state to a KILR/EILD heterodimer is -8.7 kcals/mol. Using the well characterized homodimeric coiled coil GCN4 as a template, the sequence design attempted to create coiled-coil forming proteins which bind preferentially to form heterodimers. ([http://www.ncbi.nlm.nih.gov/sites/entrez PMID:12459719 ])

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]