Coding

Part:BBa_M10035

Designed by: Sadao Ota   Group: Berkeley BioE140L - S09   (2009-05-10)

{˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

This part consists of an actual transporter portion and also passenger domain of an antigen 43 autotransporter display system based on this reference: PMID: 12107137.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal PstI site found at 1114
    Illegal PstI site found at 1385
    Illegal PstI site found at 1438
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 913
    Illegal AgeI site found at 652
    Illegal AgeI site found at 1066
    Illegal AgeI site found at 1308
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None