Part:BBa_M10022
{˂Beta Roll˃}
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
This basic part codes for the calcium cation binding beta roll motif found in the alkaline protease of Pseudomonas aeruginosa. Calcium cations are bound to two half-sites, exhibited by the GGXGXD motifs, which are brought together in pairs of loops in the beta roll. The role of this motif is not completely elucidated, but it is suspected that it affects folding after transmembrane translocation. Since the beta roll binds calcium internally, the absence of Ca2+ ions may cause instability in the fold structure, thus facilitating membrane translocation in an unfolded conformation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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