Part:BBa_M10021

{˂Poly_bind˃} passenger protein

This part encodes a passenger protein that binds to polystyrene. The synthetic peptide FKFWLYEHVIRG was reverse translated and designed to be expressed in E. coli. The start and stop codons were removed, and the BamHI, BglII, and EcoRI sites were added, along with a 5 bp cap and tail.

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

References

Feng, Bo, Youzhi Dai, Lu Wang, Nengguo Tao, Shirong Huang, and Hongyan Zeng. "A novel affinity ligand for polystyrene surface from a phage display random." Journal of the International Association for Biologicals (2009): 48-54. Pubmed. <http://pubmed.gov>. (PMID: 19056299)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]