Part:BBa_M10020

{˂TraT˃} C-terminal Display Protein

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This part encodes a C-terminal display protein. The TraT gene is part of the F sex factor of E. coli and encodes an outer membrane lipoprotein involved with cell-surface interactions.

The TraT CDS is 735 bp long. To construct the part, the prepro sequence was removed (from the beginning to the 63bp), along with the start and stop codon. Finally, the BamHI, BglII, and EcoRI sites along with a 5 bp cap and tail were added.

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

References

Chang, Hsin-Hou, and Szecheng J. Lo. "Modification with a phosphorylation tag of PKA in the." Journal of Biotechnology (2000): 115-22. Pubmed. <http://pubmed.gov>. (PMID: 9933744 )

Chang, Hsin-Hou, Shih Yi Sheu, and Szecheng J. Lo. "Expression of Foreign Antigens on the." Journal of Biomedical Science (1999): 64-70. Pubmed. <http://pubmed.gov>. (PMID: 10725535 )

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 103
  Illegal PstI site found at 142
  Illegal PstI site found at 401
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 103
  Illegal PstI site found at 142
  Illegal PstI site found at 401
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 103
  Illegal PstI site found at 142
  Illegal PstI site found at 401
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 103
  Illegal PstI site found at 142
  Illegal PstI site found at 401
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 579
  Illegal BsaI.rc site found at 61