Part:BBa_M10014

{˂eCPX!˃} - circularly permuted OmpX

OmpX stands for outer membrane protein X, whic in Escherichia coli promotes bacterial adhesion [1]. Circularly permuting OmpX such that both the N and C termini peptides are displayed on the surface improved the protein's ability to localize to the outer membrane [2]. The bacterial display scaffold was further improved by using various linkers between the N and C termini of OmpX to develop an enhanced CPX (eCPX) to optimize activity of the enhanced the efficiency

A bacterial display scaffold was recently developed which allows the display of both the N and C termini peptides of OmpX. Various linkers were used between the N and C termini of OmpX to determine the best variant (an enhanced CPX = eCPX) which optimized the protein's activity [3].

References

[1] Vogt, J., and G. E. Schulz. "The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence." Structure 10th ser. 15 (1999): 1301-309. PMID: 10545325

[2] Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands Jeffrey J. Rice, Aaron Schohn, Paul H. Bessette, Kevin T. Boulware, and Patrick S. Daugherty Protein Sci. 2006 April; 15(4): 825–836. doi: 10.1110/ps.051897806. PMCID: PMC2242469

[3] Rice, J. J., and P. S. Daugherty. "Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides." Protein Engineering, design & selection 21 (2000): 435-42. PMCID: PMC2427320

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 184
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 184
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 184
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 184
  
• 1000
  COMPATIBLE WITH RFC[1000]