Coding

Part:BBa_M10011

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-01)


{˂CPG_L6!}

Circularly permutated OmpG with a backbone opening in loop 6 of the native protein.

Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.

CPG_L6 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {<CPG_L6!} and should be functionally displayed on the outer membrane of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a{˂CPG_L6!}