Generator

Part:BBa_K988002

Designed by: Isaac Ward   Group: iGEM13_Lethbridge_Canada   (2013-06-21)


Oxytocin-Neurophysin1

This Part was developed from the Homosapien sapien (human) coding sequence for Oxytocin-Neurophysin1(1) the original signal sequence was replaced with a new (PelB) signal sequence (2) to send the protein to periplasmic space on and then be cleaved the 5' end this also allows for potential increased stability of the folded protein. A 6x-His tag was added for purification on the 3' end.

(1)http://www.uniprot.org/uniprot/P01178 (2)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259416/ (3)http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063442


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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